Brawijaya Linux User NGalam
Laboratorium Sentral Ilmu Hayati (LSIH)
Universitas Brawijaya sebagai salah satu Reasearch University/Interpreneur University dalam pembangunan IPTEK juga tidak terlepas dari tuntutan perubahan. Dengan demikian diperlukan inovasi dan ide-ide kreatif, peningkatan fasilitas dan kualitas sumber daya manusia, laboratorium yang memadai sesuai dengan perkembangan dan tuntutan jaman yang sekiranya akan lebih bermanfaat dan berdaya guna.
Berbagai kegiatan penelitian yang bersifat kompetitif yang dipercayakan kepada staf dan peneliti di lingkungan Universitas Brawijaya semakin meningkat, meliputi penelitian-penelitian Hibah Bersaing, Hibah Pasca, RusNas, Fundamental Research, Program Insentif: (a) Insentif Riset Dasar, (b) Insentif Riset Terapan, (c) Insentif Peningkatan Difusi dan Pemanfaatan IPTEK Sistem Produksi, (d) Insentif Percepatan Difusi dan Pemanfaatan IPTEK, dan (e) Insentif Riset Unggulan Strategis Nasional, Litbang Pertanian, LitBangKes, Risbin Iptekdok dan lain-lain. Arah dari program penelitian tersebut di atas cenderung bersifat multidisiplin sehingga menuntut suatu kerjasama yang baik untuk bisa menyelesaikan permasalaan secara tuntas.
Permasalahan yang ada sementara ini adalah fasilitas dan sarana laboratorium pendukung penelitian tidak lengkap dan terpisah di berbagai fakultas dan masih belum menunjukkan kinerja optimal karena sering menghadapi birokrasi administrasi antar Fakultas/Jurusan/Laboratorium yang kadang-kadang masih membatasi kolaborasi kegiatan penelitian interdisipliner. Di sisi lain fakta menunjukkan bahwa maintenance peralatan tertentu sangat mahal bila dikelola oleh laboratorium di tingkat Jurusan atau Fakultas, akibat manajemen peralatan yang tidak tertata dengan baik menjadikan alat terbengkalai, rusak karena tidak terpakai atau tidak terawat dengan baik sehingga penggunaannya menjadi tidak optimal.
Berdasarkan kondisi di atas, maka dipandang perlu dibuat suatu Laboratorium Sentral yang bertugas mengkoordinasikan dan mengakomodasikan manajemen laboratorium-laboratorium yang bersifat sentralistik dan terpadu pada tingkat Universitas Brawijaya
Visi dan Misi
Program Jangka Panjang
Program Jangka Pendek
1. Confocal Laser Scanning Microscope
This laser scanning microscope is kind of fluorescence microscopy that equipped with software to analysis a microscopic image. The fluorescence object can be sliced in several pieces until deep 20 µm by computerized processing so make an object looks 3-dimension. The specific applications of this are FRET (Fluorescence Resonance Energy Transfer) analysis for mitochondrial protein association (such as apoptosis), molecular interactions (such as Ca2+-calmodulin and ligand-receptor interactions), proten conformational changes, ion concentration, dissociation or hydrolysis, fusion of membrane-like lipid vesicles, molecules colocalizes, and bleaching method for study dynamics of phospholipids membrane in living organisms.
2. DNA Sequencer
DNA sequencer is to sequencing nucleotide arrangement from submitted bacterial cultures, plasmids, PCR products or other templates, and sequence analysis on reactions completed by users with the dye terminator chemistry
3. Real Time PCR
The basic goal of real-time PCR is to precisely distinguish and measure specific nucleic acid sequences in a sample even if there is only a very small quantity. Real-time PCR amplifies a specific target sequence in a sample then monitors the amplification progress using fluorescent technology.
4. PCR System
PCR (Polymerization chain Reaction) is a procedure by which DNA can be copied and amplified. PCR exploits DNA polymerases to amplify specific pieces of DNA using short, sequence-specific oligonucleotides added to the reaction to act as primers. PCR-based strategies have propelled molecular biology forward by enabling researchers to manipulate DNA more easily, thereby facilitating both common procedures, such as cloning, and huge endeavors such as the Human Genome Project.
5. NanoDrop Spectrophotometer
NanoDrop allows micro-quantitation of only 1 µL of sample with remarkable reproducibility. The user simply pipettes a 1 µL sample directly onto a fiber optic measurement surface where it is held in place by surface tension, eliminating the need for cuvettes or capillaries. Contain of software with nine modules: nucleic acid, microarray, protein A280, protein & labels, Protein BCA, Protein Bradford, Protein Lowry, the Number of Cell in Cultures and general UV-Vis measurement.
6. NanoDrop Fluorospectrometer
A 1-2 µl sample is pipetted directly onto the measurement surface and held in place by surface tension. Broad excitation range occurs from one of 3 LED sources: UV, blue or white. Applications for determine concentration of dsDNA (using the PicoGreen assay, Quant-iTTM DNA assay, Hoechst 33258 dye, RNA using RiboGreen dye), protein concentration using Quant-iTTM Protein Assay Kit, other additional preconfigured applications include FITC (fluorescein), Cy-Alexa Fluor dyes, B-Phycoerythrin, Quinine Sulfate, Sulforhodamine, 4-MU.
The cryotome was designed for cutting frozen sections of fixed and unfixed specimen, rapidly and accurately, for future examination by microscope. It operates by rapidly freezing the sample in a temperature controlled environment, and provide means for sectioning the frozen sample by conventional microtome. It is very convenience for prepare heat-sensitive sections i.e. lipid and enzyme.
8. Fluorescence Stereomicroscope
This kind of stereomicroscope enables even & overwhelmingly bright fluorescence observation from low to high magnifications. Its optical system provides sharp 3D observation images of various specimens and a variety of tasks including manipulation of thick specimens like eggs or embryos. 3D observation images from surface to interior are particularly effective in the dissection & manipulation of live specimens.
9. Microscope Inverted and Micromanipulator System
This system is used for in Vitro fertilization, patch clamping injection. Modern standards in easy-to operate micromanipulators vary widely according to differences of research and operation. The specially selected adapter, a pair of motorized positioners, drop handle joysticks micromanipulators and universal joints (UT-D) are combined here as a set.
10. Microscope Digital Camera 12,5 MP Model
This camera rapidly delivers images with highly accurate color along with a sharp, vivid, real-time display. The high-sensitivity, high–resolution performance is complemented with advanced, easy-to-use multifunctional software that provides powerful support for a broad range of microscope imaging requirements.
11. Horizontal & Submarine Gel Electrophoresis
The horizontal gel electrophoresis is used for separation of DNA and RNA.
12. Vertical Electrophoresis and Vertical Electrophoresis Multi gel
This system is used to separation protein SDS PAGE, Native PAGE (for isoenzyme analysis) Isoelectro Focusing (IEF) for pH isoelectric base analysed of protein, 2D electrophoresis, and nucleotide base of DNA.
13. Semi Dry Transfer System
This system is used to transfer macromolecules from gel to membrane: nitrocellulose and PVDF for protein and Nylon for DNA & RNA. This procedure is a step on immunobloting or Western Blot (for protein), Southern Blot (for DNA), and Northern Blot (for RNA) analysis.
14. Bench top Ultracentrifuge 150,000 rpm
This ultracentrifuge enables to handling volumes from as little as 175 µL to 108 mL. This perform in less time, more separations, such as sub cellular particles viruses, protein purification and isolation, lipoprotein separation, high-purity plasmid DNA, and virus isolation. This versatile model operates with maximum speed: 150,000 RPM, maximum force 1,019,000 RCFxg, set temperature range: 0 to 400C.
15. Protein or ELISA Visible Plate Reader for 96-Well Plate
This system was pre-programmed with plate set-up, standard curve and analysis software for all Biotrak Assays with colorimetric end point including EIA, ELISA and activity assay formats. Raw data or transformed results can be stored, printed, or sent to a PC for further analysis.
16 Bench top Centrifuges
This centrifuges can skip steps in cell culturing (eliminate 15 mL or 50 mL, conical tubes, centrifuge cells directly in T-75 cm2 or T-25 cm2 flasks). This system has faster separations in 4x750 mL swinging bucket rotor, with maximum speed 5,250xg. Some applications include: filtration and concentration, fast and slow blood component spins, microbial cell harvesting (up to 3 litters in just 14 minutes)
17. Mini Centrifuge
Includes micro tube and PCR strip tube rotors, includes 0.4 – 0.5 ml tube adaptors and maximum speed 6,000 rpm (2,000 x g).
18. Refrigerated Centrifuge
The centrifuge will be capable of maintaining a rotor chamber temperature of +4 degrees C. During launch and landing, the RC shall be rack mounted in an 12 PU active drawer. During on-orbit operations, the RC shall be rack mounted in an HRF rack 12 PU active drawer.
Electrophoration is a powerful, highly efficient technique for introducing nucleotides, DNA, RNA, proteins, carbohydrate, dyes, and viral particles into prokaryotic and eukaryotic cells. A high-intensity electric field transiently permeabilizes the membrane, enabling uptake of molecules from surrounding medium.
20. Deep Freezer
Special execution internal evaporator to ensure temperature stability in the freezer (+/-2°C thermal gradient). Microprocessor controlled temperature regulation system with door mounting. Insulated inner doors in thermo format. Temperature -85°C.
21. GC Analyzer
It’s easily configured to perform on-line analyses for monitoring critical gas and liquid process streams.
HPLC is a form of column chromatography used to separate components of mixture using a variety of chemical interactions between substances and chromatography column. Sometimes referred high pressure liquid chromatography.
It is provides exceptional sensitivity in an incredibly compact instrument. The bright optical system and noise suppression digital filter allow for high sensitivity measurement. Measurement wavelength range: 220-750 nm and 0 order as standard, 220-900 nm and 0 order optionally.
24. Ice Maker
This item is a kind of insulated storage compartment that make ice cubes anytime. Just plug in and add water. Starts making ice in 6 to 8 minutes. Uses tap water or bottled water. Makes 3 different size ice cubes. Makes up to 29 pounds of ice cubes per day. Stores up to 2.5 pounds of ice. No installation required. Easy to use and fully automatic.
25. Laboratory Fume Cupboard & Laminar Airflow
Thermo-controller has following functions: auto-tuning, solid state-proportional control and limitation of setting temperature. Aluminium block is used for good conduction, cleanness and convenience. Energy block is thick and touched with sample block accurately and evenly for a good conduction of heat.
27. Prime & Simples Protein Purification System
Simple purification system for antibody monoclonal, native and recombinant protein, and other small molecules with real time monitoring.
They roll right to your work bench to give you fast, effective sterilization of media, glassware, instruments, and disposables-on the spot and on your schedule. These functional autoclaves offer three cycles of operation ranging from basic sterilization cycles to automatic warming cycles. Chamber capacity 53-liters with operating temperature 105-1320C.
29. Shaking Water Bath
Temperature range from room temperature to 100 şC, haemostatic control with an accuracy of ± 0.5şC. Shaking speed range 40 to 140rpm. Built-in L-shape thermometer. To work on 220/230 volts A.C. Supply. Without RPM indicator.
30. Ultrasonic Disrupter
This ultrasonic disrupter use microprocessor control to make sonicating easier. Compact and easy to operate, capable of performing a wide variety of ultrasonic processing applications quickly and thoroughly. This versatile unit can handle volumes from 0.2 to 1000 ml. by just changing the probe. This ultrasonic processor offer programmability, auto tuning, temperature monitoring and the ability to control an external cooling device in one complete unit.
31. Cold Room
Room temperature 4-160C. Cupboard temperature
2-40C. Cold room for protein preparation & analysis.
32. SingleCompact Heating Thermomixer
To incubated sample DNA/RNA/protein and other solution at fixed temperature with shaking mode.
33. Controller Bench Top Orbital Shaker
This shaker is ideal for any shaking application for microbiology cell biology, biochemistry, and molecular biology field.
34. ChemiDoc Gel Imaging
This system is a kind of imaging instruments for basic gel documentation to sensitive chemiluminescence’s detection and high-resolution 2D gel imaging. It supports a wide variety of applications, including: nucleic acid detection, Western blotting, 2-D gel electrophoresis, dot blotting, densitometry and colony counting.
35. 2-D Electrophoresis Tetra Cells
Vertical electrophoresis has applications in all areas of research, and offers a powerful and diverse selection of protein and nucleic acid analytical techniques. Analytical protein electrophoresis is used for applications ranging from purity assessments to analysis of complex protein mixtures. It is also used for some nucleic acid applications.
36. Laboratory Glassware includes:
beaker glass, Erlenmeyer flask, measuring cylinders, volumetric flask, laboratory bottles, mortar plus pestle, alcohol burner, funnels AR-glass, pi-pump and other facilities.
Daftar Nama Pengurus
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